Primary neurons, stem cells, cell sorting, organotypic slices... | |
Preparation and cultivation of primary neurons from hippocampus, cortex, and spinal cord | | |
Preparation and culture of primary neurons | | |
Isolation and in vitro culture of neural stem cells | | |
Basic characterization of neural derivatives from iPSCs to 2D and 3D cultures | | |
The secrets of human glioma stem cell cultivation | | |
Flow cytometry - from basics to applications | | |
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Comparison of human and mouse sensory neuron in vitro (2-3 days each spring; contact us prior to March if you are interested) | | |
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Cell infection, transfection, transgenesis | |
Design of rAAV vectors and demonstration of intracranial injections | | |
Viral methods in neuroendocrinology and neuroscience | | |
Viral gene transfer and stereotaxic delivery into the rodent brain, 3D immunohistochemistry, microscopy and analysis | | |
In utero electroporation, in vitro neurovascular assay, immunohistochemistry analysis | | |
Drosophila molecular and classical genetics | | |
Lentiviral Production and Generation of Stable Tumor Cell Models | | |
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Electrophysiology | |
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Electrophysiology – Intracellular current clamp recordings in Drosophila | | |
In vitro and in vivo electrophysiological recording in the hippocampus | | |
Paired recordings from connected neurons in brain slices | | |
Whole-cell patch-clamp recordings | | |
Whole-cell patch-clamp recordings in brain slices using infrared video microscopy | | |
Electrophysiological analysis of synaptic function and small oscillating networks. Methods: Patch clamp, intracellular recording, extracellular recording, tetrodes, in vivo recording, advanced data analysis. | | |
Extracellular recordings in behaving animals | | |
Multi-electrode array (MEA) and whole-cell patch-clamp recordings of human-induced pluripotent stem cell (hiPSC)-derived neurons | | |
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In situ hybridization | |
Non-radiocative in situ mRNA hybridisation - RNAscope | | |
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Imaging | |
Video-imaging of neuronal cultures | | |
Video-imaging of neuron cultures | | |
Functional Imaging – calcium and FM 1-43 | | |
Confocal laser scanning microscopy | | |
Confocal laser scanning microscopy | | |
Immunohistochemistry and confocal imaging | | |
In vivo two-photon imaging | | |
dSTORM superresolution microscopy | | |
Transmission electron microscopy | | |
Transmission electron microscopy | | |
3D scanning electron microscopy | | |
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Behaviour | |
Automation of behavioral experiments for rodents | | |
Intravenous self-administration in rodents to model behaviors relevant to drug dependence in humans | | |
Methods in behavioural animal neuroscience | | |
Associative learning and memory | | |
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Other | |
Data analysis in neuroscience | | |
Neural data analysis practical (Prerequisite: BCCN-lecture) | | |
Transcranial magnetic stimulation in the assessment and modulation of human perception | | |
Regenerative approaches for spinal cord injury | | |
Programming for neuroscientists | | |
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Neural systems computer simulation practical (Prerequisite: BCCN-lecture) | | |
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Soft Skill Courses (NOT accepted as practical courses) | |
From the Idea to a Paper: Designing, Executing and Publishing Research in Systems Neuroscience | | |
The art of the short communication | | |
6Rs in neuroscience research: How to register, be robust and report | | |
Good scientific practice, etc. | | |